Uhrf1 abcam
WebAbcam - antibodies and reagents supplier, find any antibody Results for "uhrf1" Abcam: antibodies, proteins, kits... Your browser does not have JavaScript enabled and some … WebUhrf1 flox/flox mice were generated by embryonic stem cell (ESC) targeting and blastocyst injection at the Shanghai Research Center for Model Organisms as reported previously . Stra8-Cre mice in a C57BL/6 J background were purchased from the Jackson Laboratory. Ngn3-Cre mice were generously gifted by Prof. Lan Ye at Nanjing Medical University.
Uhrf1 abcam
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WebThe UHRF1 (H-8) Antibody was generated using UHRF1 as the antigen. It reacts with Human, Mouse, and Rat. This antibody has been shown to work in applications such as: EIA, Immunoassay, Precipitation, ELISA, Immunofluorescence, Immunohistochemistry - fixed, Immunoprecipitation, and Western Blot. Supplier Page Product Specs Company Info … WebUHRF1 plays multiple roles in regulating DNMT1-mediated DNA methylation maintenance during DNA replication. The UHRF1 C-terminal RING finger functions as an ubiquitin E3 ligase to establish histone H3 ubiquitination at Lys18 and/or Lys23, which is subsequently recognized by DNMT1 to promote its localization onto replication foci.
Web13 Jul 2015 · UHRF1, an epigenetic regulator, has been shown to be overexpressed and to coordinate tumor suppressor gene (TSG) silencing in several cancers. In a previous study, we found that UHRF1 promoted gastric cancer (GC) invasion and metastasis. However, the role and underlying mechanism of UHRF1 in GC carcinogenesis remain largely unknown. WebKong et al. show that histone and hemimethylated DNA reader domains are critical for UHRF1 to maintain aberrant DNA methylation in colorectal cancer (CRC) cells. Blocking either domain reverses DNA hypermethylation, reactivates epigenetically silenced tumor suppressor genes, and reduces CRC oncogenic properties.
Web27 Aug 2013 · anti-UHRF1 antibody (Abcam, Cambridge, UK). UHRF1 staining is primarily localized to the nucleus. The. protein expression was visualized and classified according to. Web4 Feb 2024 · These studies suggest that PGC7 affects overall DNA methylation by regulating the binding of UHRF1 to chromatin or by altering the subcellular localization of UHRF1. However, we found that endogenous DNMT1 tended to be located in the nucleus of Pgc7 -knockdown F9 cells and that the level of ERK phosphorylation decreased significantly, but …
Web17 Mar 2014 · 江苏省肿瘤医院妇瘤科210009【摘要】目的探讨UHRF1基因在人卵巢癌细胞株OVCAR-3中的表达及其生物学意义。方法利用小干扰RNA基因沉默技术，将体外化学合成的针对UHRF1基因的siRNA转染至人卵巢癌细胞株OVCAR-3细胞；实时荧光定量PCR和Westernblot分别检测转染前后细胞UHRF1mRNA和蛋白表达水平的变化；CCK-8实验 ...
Web18 Mar 2024 · Ablation of Uhrf1 in mouse satellite cells impairs their proliferation and differentiation, leading to failed muscle regeneration. Uhrf1 -deficient myogenic cells exhibited aberrant upregulation of transcripts, including Sox9, with the reduction of DNA methylation level of their promoter and enhancer region. These findings show that Uhrf1 … grey patio blocksWebPrimary Anti-HIF-1 alpha antibody [H1alpha67] (ab1) Protein Recombinant Human HIF-1 alpha protein (ab154478) Lysate Hela-DFO treated (0.5mM, 24h) Nuclear Lysate … grey swan coffee machineWeb6 Nov 2015 · Ubiquitin‑like with plant homeodomain (PHD) and RING‑finger domain 1 (UHRF1) maintains methylation patterns following DNA replication and is expressed at high levels in various types of human cancer. UHRF1 has been identified as a novel oncogene involved in the pathogenesis of hepatocellular carcinoma. Previous studies have … grey-headed bulbulWebIn obtained Uhrf1 fl/fl AID-Cre + mice, only active B cells were depleted of Uhrf1 and thereafter referred to as Uhrf1 GCB KO mice (Kwon et al., 2008). As a transgene, AID-Cre has no effect on GC response (Pérez-García et al., 2024), therefore we used Uhrf1 fl/fl mice as the control, and they were referred to as WT or Uhrf1 GCB WT mice. grey wolf in wisconsinWeb22 Mar 2024 · After UHRF1 was interfered with or overexpressed in renal carcinoma cell lines A498 and 769-P, the relative mRNA and protein level of UHRF1 was detected by RT-qPCR and immunofluorescence. ... Abcam) was added and incubated overnight at 4°C. Fluorescent secondary antibody anti-LPO antibody (HRP) was added the next day and … grey washed chest of drawersWeb6 Jun 2024 · UHRF1 silence inhibits proliferation and migration in breast cancer cells. A and B Western blotting analysis of UHRF1 expression in MCF-7 and T47D cells exposed to siControl or siUHRF1.C and D Cell proliferation analysis was performed in MCF-7 and T47D cells transfected with siControl or siUHRF1.E and F Representative images of Edu assays … grey wolf max weightWeb25 May 2024 · Loss of function mutations at the retinoblastoma ( RB1 ) gene are associated with increased mortality, metastasis and poor therapeutic outcome in several cancers, including osteosarcoma. However, the mechanism(s) through which RB1 loss worsens clinical outcome remain to be elucidated. Ubiquitin-like with PHD and Ring Finger domains … grey shoes with brown pants