Cisplatin dna cross-linking
WebFeb 1, 2002 · To overcome this obstacle, researchers have focussed their attention on cisplatin (cis-DDP; cis-platinum (II)diamminedichloride), a cross-linking agent shown to … WebCisplatin produces DNA cross-linking and is used to treat medulloblastoma, head and neck, ovarian, germ cell, cervical, lung and bladder cancers. Cisplatin produces a …
Cisplatin dna cross-linking
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WebDec 15, 1995 · The widely used antitumor drug cis -diamminedichloroplatinum (II) (cisplatin or cis -DDP) reacts with DNA, cross-linking two purine residues through the N7 … WebCisplatin crosslinks DNA in several different ways, interfering with cell division by mitosis. The damaged DNA elicits DNA repair mechanisms, which in turn activate apoptosis when repair proves impossible.
Webfavors cisplatin cross-linking (20) with inter- and intra-DNA strands (14,17,26). Cisplatin interacts primarily with cytosine- and guanine-rich DNA regions (29) and causes cellular damage. Despite its potent antineoplastic action, cisplatin’s major side effects include nephrotoxicity (2), peripheral neuropathy (33), azoospermia (11). In mammals, WebAs we have seen from studies into the mode of action of cisplatin, covalent intrastrand cross-links can also be formed by chemical agents, such as cisplatin. A similar mechanism is believed to repair damage done to cancer cells by cisplatin, as we will see when we discuss drug resistance.
WebJan 1, 2001 · We compared the DNA cross-linking capability of CC-1065 with that of its analogs: Bizelesin, which forms DNA cross-links by direct chemical bonding, and Adozelesin, a demethoxy derivative of CC-1065. Section snippets Drugs WebJul 23, 1996 · Abstract cis-Diamminedichloroplatinum (II) (cisplatin) is a widely used anticancer drug that binds to and crosslinks DNA. The major DNA adduct of the drug results from coordination of two adjacent guanine bases to platinum to form the intrastrand crosslink cis- [Pt (NH3)2 [d (GpG)-N7 (1), -N7 (2)]] (cis-Pt-GG).
WebJul 8, 2009 · The two opposing guanine residues involved in the cross-link were identified via limited alkaline hydrolysis. The kinetics of aquated cisplatin binding to the BBD RNA, a related RNA hairpin, and its DNA hairpin analogue were investigated in an ionic background of 0.1 M NaNO (3) and 1 mM Mg (NO (3)) (2).
WebCisplatin crosslinks DNA in several different ways, interfering with cell division by mitosis. The damaged DNA elicits DNA repair mechanisms, which in turn activate apoptosis … grand chapter of oklahoma oesWebPhoto-cross-linking is thus an effective tool for investigating the interaction of cisplatin-modified DNA with damage-recognition proteins under heterogeneous conditions such … chinese baitcasting reelsWebApr 14, 2024 · Among the extracellular vesicles, apoptotic bodies (ABs) are only formed during the apoptosis and perform a relevant role in the pathogenesis of different diseases. Recently, it has been demonstrated that ABs from human renal proximal tubular HK-2 cells, either induced by cisplatin or by UV light, can lead to further apoptotic death in … grand chapter of quebecWebThis chapter deals with the interstrand cross-links in cisplatin- or transplatin-modified DNA and focuses on three main aspects: the formation of the interstrand cross-links, the … grand chapter of oregonWebThe drug reacts with nucleophilic sites in DNA forming monoadducts as well as intra- and interstrand crosslinks. DNA-cisplatin adducts are specifically recognized by several … chinese baked goods onlineWebFeb 28, 2024 · Indeed, while cisplatin probably undergoes an efflux effect, compound 19 shows a time-dependent accumulation in cells and a greater ability to cross the membrane. DNA interaction has been confirmed for both complexes due to the presence of the triphenylphosphine hydrophobic moiety and the trans geometry of bromide groups. grand chapter of pa order of eastern starThe planar compound links to nucleobases through water displacement of one or both of its chloride groups, allowing cisplatin to form monoadducts to DNA or RNA, intrastrand DNA crosslinks, interstrand DNA crosslinks, and DNA-protein crosslinks. When cisplatin generates DNA crosslinks, it more frequently … See more In genetics, crosslinking of DNA occurs when various exogenous or endogenous agents react with two nucleotides of DNA, forming a covalent linkage between them. This crosslink can occur within the same strand (intrastrand) … See more Crosslinking of DNA and protein Biochemical interaction methods DNA-protein crosslinking can be caused by a variety of chemical and physical agents, including … See more Many characterized crosslinking agents have two independently reactive groups within the same molecule, each of which is able to bind with a nucleotide residue of DNA. These agents … See more Crosslinked DNA is repaired in cells by a combination of enzymes and other factors from the nucleotide excision repair (NER) pathway, See more • PDB: 1AIO – Interactive structure for cisplatin and DNA adduct formation • PDB: 204D – Interactive structure for psoralen and crosslinked DNA See more grand chapter of royal arch masons of maine