WebFeb 11, 2024 · Lactate production was measured using a Lactate Colorimetric Assay Kit II (BioVision, K627–100) according to the manufacturer’s protocol. Briefly, at 36 h post-transfection, phenol red–free RPMI 1640 medium without FBS was added to a 6-well plate of subconfluent cells and cultured for 4 h. A standard curve of nmol/well versus the … Webwww.biovision.com [email protected] Page 1 of 2 For research use only Lactate Colorimetric Assay Kit II (Catalog #K627-100; 100 assays; Store kit at –20°C) I. …
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WebComponents 100 assays Cap Color Part Number Lactate Assay Buffer Lactate Enzyme Mix Lactate Substrate Mix L(+)-Lactate Standard (100 mM) 25 ml lyophilized lyophilized 100 … WebJan 15, 2024 · Then, the media were collected for lactate production assay. L-(+)-Lactate was measured with a colorimetric kit (BioVision, K627-100) according to the manufacturer’s instructions. Absorbance (optical density 450 nm) for each sample was background corrected with the culture medium (2% FBS) collected from the well without growing cells. ... crystal egyptian rice
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WebBioVision rev. 06/11 BioVision Incorporated Tel: 408-493-1800 Fax: 408-493-1801 155 S. Milpitas Boulevard, Milpitas, CA 95035 USA www.biovision.com tech@ ... K627 … WebK620-100-1 K620-100-2A K620-100-4 K620-100-5 III. Reagent Preparation: subtracted from sa Ethanol Probe: Ready to use as supplied. Warm to room temperature prior to use. Store at –20oC, avoid contamination with water, protect from light. Use within two months. Ethanol Enzyme Mix: Add 220 l Ethanol Assay Buffer to the Ethanol Enzyme Mix and WebSep 1, 2014 · Culture medium was collected at day 1, day 3 and day 7. The glucose and lactate concentration within the medium were determined by Glucose Colorimetric Assay Kit (Biovision, K686-100, Milpitas, CA, USA) and Lactate Colorimetric Assay Kit (Biovision, K627-100) respectively. dwayne armein brown