WebMar 6, 2014 · Plasmids with high similarity to LFS gene or to bulb alliinase gene fragment were selected for labeling with the Biotin Nick Translation Mix (Roche Diagnostics Gmbh, Mannheim, Germany). 5S rDNA A plasmid carrying the 5S rRNA gene of rye (pScT7, [ 31 ] was labeled by Biotin-16-dUTP using Biotin- Nick Translation Mix according to the … WebNov 5, 2024 · FISH was performed using the cloned satDNA sequences as probes after they were labeled by nick-translation with digoxigenin-11-dUTP (DIG-Nick Translation mix, Roche Applied Science) for SATCHO1 ...
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WebJan 1, 2015 · Nick translation: Reaction mixtures for probe labeling can be purchased, e.g., DIG-nick translation mix (catalog no. 11-745-816-910) and Biotin–nick translation mix (catalog no. 11-745-824-910) from Roche Applied Science. High-yield and good-quality probes can be harvested using the manufacturer’s instructions. WebRoche. 10976776001. Nick Translation Kit. sufficient for 50 labeling reactions, kit of 1 (7 components), suitable for FISH. View Price and Availability. Roche. ... for highly sensitive filter hybridization probes, we … ed helms quotes
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WebRoche biotin nick translation mix kit Biotin Nick Translation Mix Kit, supplied by Roche, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more WebDescription: Biotin16 Nick Translation Labeling Kit contains all reagents (except template and materials for purification of the probe) required for nick translation-based Biotin … Webwww.roche-applied-science.com Figure 4: Dorsal view of a zebrafish embryo whole mount in situ hybridization. The slide was stained with a ... Biotin-Nick Translation Mix* 11 745 824 910 160 μl Nucleotides for Labeling Digoxigenin-11-dUTP, alkali-labile 11 573 152 910 11 573 179 910 25 nmol (25 μl) connected smart dishwasher