Bioline cdna synthesis kit
WebExcellent results from a variety of samples. Genomic DNA was isolated in duplicate from E. coli, mouse tail, mouse lung, HeLa cells and 3T3 cells, using ISOLATE II Genomic DNA Kit (lanes 1-5 and 6-10 respectively) …
Bioline cdna synthesis kit
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WebJan 31, 2013 · cDNA synthesis was performed with Tetro Reverse Trancriptase kit (Bioline Ltd.) according to the manufacturer’s recommendation. Quantitative PCR was performed using real-time PCR (ABI PRISM 7900, Applied Biosystems). ... reverse transcription reaction using the designed miRNA specific stem loop-RT primer and … WebThe polymerase (catalog no. BIO-21105, Bioline, London, UK) was RNA was then converted into the first-strand cDNA using an used for the second-round amplification. The cycling conditions oligo-dT primer and a Tetro cDNA synthesis kit (catalog no. BIO- were initial denaturation at 95°C for 3 min, followed by 40 cycles 65043, Bioline, London ...
WebmRNA expression was assessed by quantitative RT-PCR (q RT-PCR) using SensiFAST™ SYBR ® Lo-ROX Kit (Bioline/Meridian, Memphis, TN, USA), as described previously . Briefly, total RNA was isolated from cell lysates using the RNeasy mini kit (Qiagen, Hilden, Germany), followed by the preparation of cDNA using the SensiFAST TM cDNA … WebTo complement the SensiFAST™ Probe and SYBR® kits, Meridian has developed the SensiFAST cDNA Synthesis Kit. Product Highlights. Efficient – high-target affinity, …
WebSensiFAST™ cDNA Synthesis Kit has been developed to generate high-quality first strand cDNA from total RNA template. SensiFAST™ cDNA Synthesis Kit provides a rapid and … WebThe Tetro cDNA Synthesis Kit is optimized for RT reactions using a wide range of total RNA amounts (100pg-2_g), such that long and low abundance cDNAs can be detected …
WebFor RT-PCR, RNA-seq, and other applications, LunaScript RT Master Mix Kit (Primer-free) ( NEB #E3025) is an optimized master mix containing all the necessary components for first strand cDNA synthesis, except for primers. The mix is compatible with random primers, oligo dT primers, and gene-specific primers, enabling maximum cDNA synthesis ...
WebThe synthesis of cDNA was performed as per instructions from the SensiFASTTM cDNA Synthesis kit (Bioline). The cDNA and targeted gene primers were mixed with dNTPs, buffer, and SYBR Green Master Mix (Thermo Fisher Scientific, AU) in a 386-well plate at 10 μL per well as protocol indicated. Forty cycles of real-time qPCR were detected by a ... how is maruchan madeWebApr 13, 2024 · Lipofectamine 2000, Tetro cDNA synthesis kit and QuickExtract DNA extraction were ordered from Invitrogen (USA), Bioline (Germany), and Lucigen (USA), respectively. Primary and secondary antibodies, and fluorescence-tagged secondary antibodies were purchased from Santa Cruz Biotechnology (USA). Midorigreen was … how is martin luther king jr remembered todayWebContent And Storage. Contents: The kit includes reagents and buffers for 10 first- and second-strand cDNA synthesis reactions converting 25-50 µg total RNA or 0.2-5 µg mRNA into double-stranded cDNA. Storage: Store … highlands county webeocWebProduct Description. Tetro™ cDNA Synthesis Kit contains our highly sensitive MMLV reverse transcriptase, oligo (dT)18 and random hexamer primers and all the necessary components to generate high quality cDNA from RNA templates (fig. 1). The first-strand cDNA generated is ideal for PCR (fig. 2) and can be used in a variety of other ... how is marv alive in sin city 2WebEasyScript Plus™ cDNA Synthesis Kit is a complete system for efficient synthesis of first strand cDNA from RNA templates with secondary structure and high GC contents. The kit utilizes a special Reverse Transcriptase, the EasyScript Plus™, which is a novel recombinant reverse transcriptase that exhibits much higher efficiency in the first strand … how is marvel doingWebApr 6, 2024 · Proper, tissue-specific regulation of CYP19, the gene encoding aromatase, the key enzyme of estrogen synthesis, is essential for reproductive processes. Here, we analyzed transcriptional regulation of the porcine CYP19 in female and male gonads and brain by 5’RACE and RT-PCR and comprehensively mapped the pig CYP19 locus by in … how is martin roberts nowWebBioline Cdna found in: SensiFAST™ cDNA Synthesis Kit, Tetro™ cDNA Synthesis Kit, ISOLATE II RNA Plant Kit, Random Hexamer Primers, SensiMix™ SYBR® &.. highlands coventry ri